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(A) Morphology of MDA-MB-435s and MDA-MB-231 cells transiently transfected with mature miR-7 or the control miRNA. (B) Immunoblot analysis of FIBRONECTIN, <t>VIMENTIN,</t> <t>E-CADHERIN,</t> N-CADHERIN and <t>SNAIL</t> in MDA-MB-435s and MDA-MB-231 cells transfected with mature miR-7 or control miRNA. (C) The correlation of mature miR-7 with E-CADHERIN mRNA in 42 breast cancer samples. (D) Correlation of mature miR-7 with VIMENTIN mRNA in 42 breast cancer samples. Pearson correlation coefficients (R) and P-values (p) are indicated.
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(A) Morphology of MDA-MB-435s and MDA-MB-231 cells transiently transfected with mature miR-7 or the control miRNA. (B) Immunoblot analysis of FIBRONECTIN, <t>VIMENTIN,</t> <t>E-CADHERIN,</t> N-CADHERIN and <t>SNAIL</t> in MDA-MB-435s and MDA-MB-231 cells transfected with mature miR-7 or control miRNA. (C) The correlation of mature miR-7 with E-CADHERIN mRNA in 42 breast cancer samples. (D) Correlation of mature miR-7 with VIMENTIN mRNA in 42 breast cancer samples. Pearson correlation coefficients (R) and P-values (p) are indicated.
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(A) Morphology of MDA-MB-435s and MDA-MB-231 cells transiently transfected with mature miR-7 or the control miRNA. (B) Immunoblot analysis of FIBRONECTIN, <t>VIMENTIN,</t> <t>E-CADHERIN,</t> N-CADHERIN and <t>SNAIL</t> in MDA-MB-435s and MDA-MB-231 cells transfected with mature miR-7 or control miRNA. (C) The correlation of mature miR-7 with E-CADHERIN mRNA in 42 breast cancer samples. (D) Correlation of mature miR-7 with VIMENTIN mRNA in 42 breast cancer samples. Pearson correlation coefficients (R) and P-values (p) are indicated.
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(A) Morphology of MDA-MB-435s and MDA-MB-231 cells transiently transfected with mature miR-7 or the control miRNA. (B) Immunoblot analysis of FIBRONECTIN, <t>VIMENTIN,</t> <t>E-CADHERIN,</t> N-CADHERIN and <t>SNAIL</t> in MDA-MB-435s and MDA-MB-231 cells transfected with mature miR-7 or control miRNA. (C) The correlation of mature miR-7 with E-CADHERIN mRNA in 42 breast cancer samples. (D) Correlation of mature miR-7 with VIMENTIN mRNA in 42 breast cancer samples. Pearson correlation coefficients (R) and P-values (p) are indicated.
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Image Search Results


(A) Morphology of MDA-MB-435s and MDA-MB-231 cells transiently transfected with mature miR-7 or the control miRNA. (B) Immunoblot analysis of FIBRONECTIN, VIMENTIN, E-CADHERIN, N-CADHERIN and SNAIL in MDA-MB-435s and MDA-MB-231 cells transfected with mature miR-7 or control miRNA. (C) The correlation of mature miR-7 with E-CADHERIN mRNA in 42 breast cancer samples. (D) Correlation of mature miR-7 with VIMENTIN mRNA in 42 breast cancer samples. Pearson correlation coefficients (R) and P-values (p) are indicated.

Journal: PLoS ONE

Article Title: MicroRNA-7 Inhibits Epithelial-to-Mesenchymal Transition and Metastasis of Breast Cancer Cells via Targeting FAK Expression

doi: 10.1371/journal.pone.0041523

Figure Lengend Snippet: (A) Morphology of MDA-MB-435s and MDA-MB-231 cells transiently transfected with mature miR-7 or the control miRNA. (B) Immunoblot analysis of FIBRONECTIN, VIMENTIN, E-CADHERIN, N-CADHERIN and SNAIL in MDA-MB-435s and MDA-MB-231 cells transfected with mature miR-7 or control miRNA. (C) The correlation of mature miR-7 with E-CADHERIN mRNA in 42 breast cancer samples. (D) Correlation of mature miR-7 with VIMENTIN mRNA in 42 breast cancer samples. Pearson correlation coefficients (R) and P-values (p) are indicated.

Article Snippet: Proteins from total cell lysates were resolved by SDS-PAGE, transferred to the Nitrocellulose membrane (GE healthcare), blocked in 5% non-fat milk in PBS/Tween-20, and blotted with the antibodies for FAK (3258, 1∶1000, Cell Signaling Technology), phospho-FAK(Tyr 397 ) (3283, 1∶1000, Cell Signaling Technology), VIMENTIN (550513, 1∶5000, BD Pharmingen), FIBRONECTIN (610077, 1∶5000, BD Transduction Laboratories), E-CADHERIN (610181, 1∶5000, BD Transduction Laboratories), N-CADHERIN (610920, 1∶2000, BD Transduction Laboratories), SNAIL (sc-28199, 1∶1000, SantaCruz Biotechnology) and GAPDH (M20028, 1∶5000, Abmart, Shanghai, China).

Techniques: Transfection, Western Blot